The Effect of Bitter Extracts (Andrographis Paniculata Nees.) of Ovarium Histologys (Mus Musculus L.)

Abstract

Bitter is widely used in the treatment of various diseases such as fever, gastric infections, respiratory infections, malarial fever, insect repellent, diabetic complications, protects against liver diseases, antivirals, immunostimulators and suppresses retenosis in angiosplastic patients. However, its effect on the reproductive system, especially on the female reproductive system is controversial. The asrographolide isolated from bitter (A. paniculata) has anticancer activity through the mechanism of apoptosis against cancer cells HeLa.Ovarium is a very important reproductive organs in the animal reproductive system. as a producer of ova, estrogen and progesterone.

              The research was conducted at Zoology Laboratory and Animal Home Laboratory of FMIPA UNP. The design used was Completely Randomized Design with 4 treatments and 6 repetitions. The treatment used was control, given with bitoto extract of 0.2 g / BB / head / day, 0.4 g / BB / head / day, and 0.6 g / BB / head / day. The treatment was carried out for 12 days referring to the oogenesis cycle in the mouse, then made histology of each treatment and observations included measurement of ovarian weight and size, number of primary follicles, secondary follicles, tertiary follicles, De Graaf follicles, corpus luteum and abnormalities damage to the ovarian histology of mice. The data were analyzed using the Analysis of Variable Fingerprint (ANOVA), if significantly different then it will be continued with BNT advanced test with 5% level.

The results of this study showed that, giving oral administration of oral extract for 12 days did not affect the histology of the ovaries of mice statistically, but tended to have an effect on increasing the number of tertiary follicles in the ovaries of mice.